Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein

J Steyaert; L Van Melderen; P Bernard; M H Thi; R Loris; L Wyns; M Couturier

doi:10.1006/jmbi.1993.1301

Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein

J Mol Biol. 1993 May 20;231(2):513-5. doi: 10.1006/jmbi.1993.1301.

Authors

J Steyaert¹, L Van Melderen, P Bernard, M H Thi, R Loris, L Wyns, M Couturier

Affiliation

¹ Instituut Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.

PMID: 8389886
DOI: 10.1006/jmbi.1993.1301

Abstract

Large crystals of the Escherichia coli F plasmid CcdB killer protein were grown from solutions containing 32% ammonium sulphate. The crystals belong to space group P4(2)2(1)2 with a = b = 104.52 A and c = 88.45 A or P2(1)2(1)2(1) with a = 77.62 A, b = 93.28 A and c = 141.44 A. Both crystal forms diffract to 2.6 A resolution. Structure determination by multiple isomorphous replacement is under way.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry*
Bacterial Proteins / isolation & purification
Bacterial Toxins / chemistry*
Bacterial Toxins / isolation & purification
Circular Dichroism
Crystallization
Escherichia coli / chemistry*
F Factor
Topoisomerase I Inhibitors
X-Ray Diffraction

Substances

Bacterial Proteins
Bacterial Toxins
CcdB protein, Plasmid F
Topoisomerase I Inhibitors