Abstract
TGF-beta (5 ng/ml) increased the proliferation rate of HRSC by 30% after 96 h of treatment. One type of TGF-beta binding system, with apparent Kd of 953 pM and a number of 29,400 receptors/cell, was detected by Scatchard analysis of [125I]-TGF-beta binding. However, crosslinking experiments and SDS-PAGE separation showed five TGF-beta binding proteins: 50, 70, 110, 140 and 400 kDa. We may suggest that the affinities of these receptors are too close to be revealed by Scatchard plot. All together, the data suggest that TGF-beta plays a role in the hyperplasia of RA synovial tissue.
MeSH terms
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Arthritis, Rheumatoid / metabolism*
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Autoradiography
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Cell Division / drug effects
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Cells, Cultured
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Cross-Linking Reagents / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Humans
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Iodine Radioisotopes
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Kinetics
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Receptors, Cell Surface / biosynthesis*
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Receptors, Transforming Growth Factor beta
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Stimulation, Chemical
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Synovial Membrane / cytology
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Synovial Membrane / metabolism*
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Transforming Growth Factor beta / pharmacology*
Substances
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Cross-Linking Reagents
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Iodine Radioisotopes
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Receptors, Cell Surface
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta