Microheterogeneity in O-type sugar chains of carbohydrases secreted by Asp. awamori

K N Neustroev; A M Golubev; F M Ibatullin; A V Moseichuk

Microheterogeneity in O-type sugar chains of carbohydrases secreted by Asp. awamori

Biochem Mol Biol Int. 1993 May;30(1):107-13.

Authors

K N Neustroev¹, A M Golubev, F M Ibatullin, A V Moseichuk

Affiliation

¹ Dept. of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russia.

PMID: 8358322

Abstract

This paper deals with microheterogeneity in the structure of O-linked sugars of carbohydrases secreted by Asp. awamori, namely glucoamylase, alpha-galactosidase and alpha-glucosidase. Microheterogeneity was found to be related both to post-secretion deglycosylation and to changes in transferase activity induced by the differences in culturing conditions.

MeSH terms

Aspergillus / enzymology*
Aspergillus / growth & development
Carbohydrate Sequence
Culture Media
Galactose / analysis
Galactosidases / chemistry*
Galactosidases / metabolism
Glucan 1,4-alpha-Glucosidase / chemistry*
Glucan 1,4-alpha-Glucosidase / metabolism
Glucosidases / chemistry*
Glucosidases / metabolism
Glycosylation
Mannose / analysis
Molecular Sequence Data

Substances

Culture Media
Galactosidases
Glucosidases
Glucan 1,4-alpha-Glucosidase
Mannose
Galactose