Chemoenzymatic galactosialylation with integrated cofactor regeneration

P Stangier; W Treder; J Thiem

doi:10.1007/BF00731183

Chemoenzymatic galactosialylation with integrated cofactor regeneration

Glycoconj J. 1993 Feb;10(1):26-33. doi: 10.1007/BF00731183.

Authors

P Stangier¹, W Treder, J Thiem

Affiliation

¹ Institut für Organische Chemie, Universität Hamburg, Germany.

PMID: 8358224
DOI: 10.1007/BF00731183

Abstract

As a precursor for the chemical synthesis of sialylated oligosaccharides, the trisaccharide glycoside Neu5Ac alpha (2-8)Gal beta (1-4)GlcNAc beta (1-O)-pent-4-ene was synthesized starting from GlcNAc beta (1-O)-pent-4-ene, UDP-glucose and N-acetylneuraminic acid in a one pot reaction employing galactosyltransferase and alpha (2-6)sialyltransferase in a complete cofactor regeneration system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Cytidine Monophosphate N-Acetylneuraminic Acid / chemical synthesis*
Galactosyltransferases*
Molecular Sequence Data
Molecular Structure
N-Acylneuraminate Cytidylyltransferase / isolation & purification
Sialyltransferases / isolation & purification*
Trisaccharides / chemical synthesis*
beta-D-Galactoside alpha 2-6-Sialyltransferase

Substances

Trisaccharides
N-acetylneuraminyl-2-6-galactopyranosyl-1-4-N-acetylglucosaminyl-1-O-pent-4-ene
Cytidine Monophosphate N-Acetylneuraminic Acid
Galactosyltransferases
Sialyltransferases
N-Acylneuraminate Cytidylyltransferase
beta-D-Galactoside alpha 2-6-Sialyltransferase