Electrophoretic karyotyping of the lignin-degrading basidiomycete Phanerochaete chrysosporium

Mol Microbiol. 1993 May;8(5):803-7. doi: 10.1111/j.1365-2958.1993.tb01627.x.

Abstract

Electrophoretic karyotyping of the two most widely studied strains of Phanerochaete chrysosporium, BKMF-1767 and ME-446, has been determined using transverse alternating field electrophoresis. The genomic DNA of BKMF-1767 was resolved into 10 chromosomes ranging in size from 1.8-5.0 Mb, amounting to a total genome size of about 29 Mb. The genomic DNA of strain ME-446, on the other hand, was resolved into 11 chromosomes, amounting to a total genome size of about 32 Mb. Lignin peroxidase genes have been localized to five chromosomes in strain BKMF-1767 and to four chromosomes in strain ME-446.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Basidiomycota / genetics*
  • Basidiomycota / metabolism
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Fungal*
  • DNA, Fungal / genetics
  • Electrophoresis, Gel, Pulsed-Field*
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Isoenzymes / genetics*
  • Karyotyping / methods*
  • Lignin / metabolism*
  • Peroxidases / genetics*

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Isoenzymes
  • Lignin
  • Peroxidases
  • lignin peroxidase