The effect of Vitamin E treatment was studied in experimental hyperlipidaemia. The male Wistar rats got control diet and parallel fat rich diet contained 2% cholesterol, 0.5% cholic acid and 20% sunflower oil added into LATI food during 9 days. The treated hyperlipidemic animals got vitamin E for 9 days in daily 8.56 mg/b.w.kg dose mixed in food. The effect of antioxidant treatment on changes of lipid peroxidation, content of diene conjugates, thiobarbituric acid reactive products and natural scavenger capacity of rat liver homogenates and microsome fractions were measured by spectrophotometric and luminometric methods. Fatty acid composition of lipid fraction of samples was determined by capillary gas chromatography. Vitamin E in hyperlipidemia increased the natural scavenger capacity of liver and decreased the level of thiobarbituric acid reactive products and dien conjugates. There was no change in fatty acid composition of samples on effect of vitamin E antioxidant treatment.