Identification of amino acid-base contacts in the Myc-DNA complex by site-specific bromouracil mediated photocrosslinking

EMBO J. 1994 Jan 1;13(1):200-4. doi: 10.1002/j.1460-2075.1994.tb06249.x.

Abstract

Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C-3A-2C-1G+1T+2G+3-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc-DNA complex. Our results strongly suggest that homologous amino acids of Myc and Max make equivalent contacts in the respective protein-DNA complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / metabolism
  • Base Sequence
  • Binding Sites
  • Bromouracil / pharmacology
  • Cross-Linking Reagents / pharmacology
  • Crystallography, X-Ray
  • DNA / chemistry
  • DNA / metabolism*
  • Molecular Conformation
  • Molecular Sequence Data
  • Nucleotides / metabolism
  • Proto-Oncogene Proteins c-myc / chemistry
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Sequence Homology, Amino Acid

Substances

  • Amino Acids
  • Cross-Linking Reagents
  • Nucleotides
  • Proto-Oncogene Proteins c-myc
  • Bromouracil
  • DNA