Abstract
A genomic fragment with the human beta-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human beta-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I) x poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Antiviral Agents / pharmacology
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Base Sequence
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Cloning, Molecular
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Cycloheximide / pharmacology
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Gene Expression Regulation, Viral* / drug effects
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Genetic Vectors
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Humans
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Interferon-beta / biosynthesis*
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Interferon-beta / genetics*
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Mice
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Molecular Sequence Data
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Moloney murine leukemia virus / genetics
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Poly I-C / pharmacology
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Poly U / pharmacology
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RNA, Double-Stranded / pharmacology
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RNA, Messenger / analysis
Substances
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Antiviral Agents
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RNA, Double-Stranded
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RNA, Messenger
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Poly U
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Interferon-beta
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poly(I).poly(c12,U)
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Cycloheximide
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Poly I-C