Constitutive interferon expression from retroviral vector

Antiviral Res. 1993 Oct;22(2-3):215-21. doi: 10.1016/0166-3542(93)90097-3.

Abstract

A genomic fragment with the human beta-interferon gene was cloned into a pL3-4, a defective Moloney murine leukemia virus (M-MuLV) vector. Here we show that clones selected after viral infection of mouse NIH 3T3 cells constitutively produced 128 IU/ml of human beta-interferon. Constitutive synthesis of retroviral RNA was confirmed by dot blot hybridization of RNA isolated from two of the selected clones. Poly(I) x poly(C) and cycloheximide induction resulted in an increased RNA level, but this was not reflected in an increased production of biologically active interferon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Antiviral Agents / pharmacology
  • Base Sequence
  • Cloning, Molecular
  • Cycloheximide / pharmacology
  • Gene Expression Regulation, Viral* / drug effects
  • Genetic Vectors
  • Humans
  • Interferon-beta / biosynthesis*
  • Interferon-beta / genetics*
  • Mice
  • Molecular Sequence Data
  • Moloney murine leukemia virus / genetics
  • Poly I-C / pharmacology
  • Poly U / pharmacology
  • RNA, Double-Stranded / pharmacology
  • RNA, Messenger / analysis

Substances

  • Antiviral Agents
  • RNA, Double-Stranded
  • RNA, Messenger
  • Poly U
  • Interferon-beta
  • poly(I).poly(c12,U)
  • Cycloheximide
  • Poly I-C