Salmonella naestved strain AHI-195, of calf origin, harbors a conjugative 95 megadalton (MDa) plasmid, pTE195, which encodes resistance to tetracycline and chloramphenicol and belongs to incompatibility group FII. Moreover, DNA homology between pTE195 and the Salmonella dublin virulence plasmid pTE800 was revealed by digestion with several restriction endonucleases and confirmed by hybridization with different specific probes. These results indicate that pTE195 carries not only genes for drug resistance but also genes for virulence phenotypes such as serum resistance and mouse lethality.