Abstract
The influence of antigen epitope density and order on B cell induction and antibody production was assessed with the glycoprotein of vesicular stomatitis virus serotype Indiana [VSV-G (IND)]. VSV-G (IND) can be found in a highly repetitive form the envelope of VSV-IND and in a poorly organized form on the surface of infected cells. In VSV-G (IND) transgenic mice, B cells were unresponsive to the poorly organized VSV-G (IND) present as self antigen but responded promptly to the same antigen presented in the highly organized form. Thus, antigen organization influences B cell tolerance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis*
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Antibodies, Viral / immunology
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Antibody Affinity
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B-Lymphocytes / immunology*
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Immune Tolerance*
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Immunization
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Immunoglobulin G / biosynthesis
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Immunoglobulin M / biosynthesis
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Lymphocyte Activation
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Macrophages, Peritoneal / microbiology
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Membrane Glycoproteins*
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Mice
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Mice, Transgenic
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Neutralization Tests
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T-Lymphocytes, Helper-Inducer / immunology
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Vesicular stomatitis Indiana virus / immunology*
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Vesicular stomatitis Indiana virus / physiology
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Viral
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G protein, vesicular stomatitis virus
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Immunoglobulin G
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Immunoglobulin M
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Membrane Glycoproteins
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Viral Envelope Proteins