Phosphoinositides in membranes that build up the triads of rabbit skeletal muscle

FEBS Lett. 1994 May 30;345(2-3):211-8. doi: 10.1016/0014-5793(94)00440-4.

Abstract

The total membrane concentrations of PtdIns, PtdIns4P, and PtdIns(4,5)P2 contribute to the functional capacity of the Ins(1,4,5)P3 signalling system which is operating in skeletal muscle but the function of which is still unknown. Total amounts of these phosphoinositides have been determined in purified membranes of transverse tubules (TT) and terminal cisternae (TC) of the sarcoplasmic reticulum (SR) of rabbit skeletal muscle. PtdIns and PtdIns4P have been detected in both membrane systems whereas PtdIns(4,5)P2 (290 mumol/mol phospholipid) is confined only to TT. A much greater pool of PtdIns(4,5)P2 seems, however, to be located in the sarcolemma away from the triadic junction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centrifugation, Density Gradient
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Inositol 1,4,5-Trisphosphate / physiology
  • Membrane Lipids / analysis
  • Membrane Lipids / isolation & purification
  • Membrane Lipids / physiology*
  • Microsomes / metabolism*
  • Muscles / chemistry
  • Muscles / metabolism
  • Muscles / physiology*
  • Phosphatidylinositols / analysis
  • Phosphatidylinositols / isolation & purification
  • Phosphatidylinositols / physiology*
  • Rabbits
  • Signal Transduction
  • Tritium

Substances

  • Membrane Lipids
  • Phosphatidylinositols
  • Tritium
  • Inositol 1,4,5-Trisphosphate