Cloning of a family of cyclins from Arabidopsis thaliana

Biochim Biophys Acta. 1994 May 17;1218(1):115-8. doi: 10.1016/0167-4781(94)90112-0.

Abstract

Degenerate oligonucleotide primers corresponding to two conserved regions in animal cyclins were used to amplify cyclin sequences from Arabidopsis thaliana by polymerase chain reaction (PCR). Screening of a floral meristem cDNA library with two distinct PCR-generated cyclin sequences resulted in the isolation of four different cyclin cDNAs. Southern analysis of genomic DNA and sequence information from the isolated clones suggest that there is a family of cyclins in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Cyclins / genetics*
  • DNA, Complementary
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Alignment

Substances

  • Cyclins
  • DNA, Complementary

Associated data

  • GENBANK/L27223
  • GENBANK/L27224
  • GENBANK/L27225