Expression and stability of recombinant RQ-mRNAs in cell-free translation systems

V I Ugarov; Morozov IYu; G Y Jung; A B Chetverin; A S Spirin

doi:10.1016/0014-5793(94)80255-6

Expression and stability of recombinant RQ-mRNAs in cell-free translation systems

FEBS Lett. 1994 Mar 14;341(1):131-4. doi: 10.1016/0014-5793(94)80255-6.

Authors

V I Ugarov¹, Morozov IYu, G Y Jung, A B Chetverin, A S Spirin

Affiliation

¹ Institute of Protein Research, Russian Academy of Sciences, Puschino, Moscow Region.

PMID: 8137913
DOI: 10.1016/0014-5793(94)80255-6

Abstract

Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allolevivirus / genetics*
Cell-Free System
Chloramphenicol O-Acetyltransferase / antagonists & inhibitors
Cloning, Molecular
Folic Acid Antagonists
Protein Biosynthesis
RNA, Messenger / biosynthesis*
RNA, Messenger / metabolism
RNA, Viral / biosynthesis*
RNA, Viral / genetics

Substances

Folic Acid Antagonists
Qbeta ribonucleic acid
RNA, Messenger
RNA, Viral
Chloramphenicol O-Acetyltransferase