Abstract
Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Affinity Labels
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Amino Acid Sequence
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Animals
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Biological Transport
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Carrier Proteins / chemistry
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Carrier Proteins / isolation & purification*
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Carrier Proteins / metabolism
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Cell Membrane
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Cholic Acid
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Cholic Acids / metabolism*
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Electrophoresis, Gel, Two-Dimensional
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Electrophoresis, Polyacrylamide Gel
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Kinetics
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Liver / metabolism*
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Male
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Membrane Proteins / chemistry
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Membrane Proteins / isolation & purification*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Molecular Weight
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Rats
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Rats, Sprague-Dawley
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Taurocholic Acid / metabolism
Substances
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Affinity Labels
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Carrier Proteins
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Cholic Acids
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Membrane Proteins
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Taurocholic Acid
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Cholic Acid