Abstract
Putative cDNA clones for a nuclear antigen that cross-reacts with anti-human aldolase A monoclonal antibody MAb1A2 were isolated from the HeLa lambda gt11 cDNA library and a candidate clone (clone 3) was analyzed. The cDNA has an open reading frame (ORF) of 1,317 bp encoding a novel RNA helicase belonging to the DEAD RNA helicase family. The ORF also contains a nuclear targeting signal and the epitope for MAb1A2. The putative RNA helicase has sequence similarity to Escherichia coli RNA helicase DEAD, mouse translation factor eIF-4A, and human p68 and p54.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Nucleus / enzymology
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Cloning, Molecular
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DNA, Complementary / metabolism
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Escherichia coli / enzymology
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Eukaryotic Initiation Factor-4A
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Fructose-Bisphosphate Aldolase / genetics*
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Gene Library
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HeLa Cells
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Humans
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Mice
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Molecular Sequence Data
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Open Reading Frames
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Peptide Initiation Factors / genetics
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RNA Helicases
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RNA Nucleotidyltransferases / biosynthesis*
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RNA Nucleotidyltransferases / genetics*
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Peptide Initiation Factors
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Eukaryotic Initiation Factor-4A
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RNA Nucleotidyltransferases
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RNA Helicases
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Fructose-Bisphosphate Aldolase