A novel human homologue of a dead-box RNA helicase family

Biochem Biophys Res Commun. 1994 Mar 15;199(2):748-54. doi: 10.1006/bbrc.1994.1292.

Abstract

Putative cDNA clones for a nuclear antigen that cross-reacts with anti-human aldolase A monoclonal antibody MAb1A2 were isolated from the HeLa lambda gt11 cDNA library and a candidate clone (clone 3) was analyzed. The cDNA has an open reading frame (ORF) of 1,317 bp encoding a novel RNA helicase belonging to the DEAD RNA helicase family. The ORF also contains a nuclear targeting signal and the epitope for MAb1A2. The putative RNA helicase has sequence similarity to Escherichia coli RNA helicase DEAD, mouse translation factor eIF-4A, and human p68 and p54.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Nucleus / enzymology
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Escherichia coli / enzymology
  • Eukaryotic Initiation Factor-4A
  • Fructose-Bisphosphate Aldolase / genetics*
  • Gene Library
  • HeLa Cells
  • Humans
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames
  • Peptide Initiation Factors / genetics
  • RNA Helicases
  • RNA Nucleotidyltransferases / biosynthesis*
  • RNA Nucleotidyltransferases / genetics*
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Peptide Initiation Factors
  • Eukaryotic Initiation Factor-4A
  • RNA Nucleotidyltransferases
  • RNA Helicases
  • Fructose-Bisphosphate Aldolase

Associated data

  • GENBANK/D26528