Abstract
We cloned and characterized two allelic variants of the gene for the Bombyx mori prothoracicotropic hormone (PTTH), a homodimeric 30-kDa brain secretory protein. These PTTH genes contain five exons that encode a precursor protein consisting of 224 amino acid residues whose C-terminal 109 residues represent the PTTH subunit. The Bombyx haploid genome contains a single copy of the PTTH gene. The major site of PTTH expression is the brain but expression at a very low level occurs in the gut. One Bombyx brain at day 0 of the fifth larval instar contained 2.4-2.8 pg PTTH mRNA, and this amount did not change markedly during larval-pupal development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Bombyx / genetics*
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Brain / growth & development
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Brain / metabolism
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Cloning, Molecular
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DNA / isolation & purification
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DNA / metabolism
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DNA Primers
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Gene Expression
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Genes, Insect*
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Genomic Library
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Insect Hormones / biosynthesis
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Insect Hormones / genetics*
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Macromolecular Substances
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Molecular Sequence Data
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Neuropeptides / genetics
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Oligonucleotide Probes
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Restriction Mapping
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Transcription, Genetic
Substances
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DNA Primers
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Insect Hormones
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Macromolecular Substances
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Neuropeptides
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Oligonucleotide Probes
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RNA, Messenger
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prothoracicotropic hormone
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DNA
Associated data
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GENBANK/X75941
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GENBANK/X75942