Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli

H Bergler; P Wallner; A Ebeling; B Leitinger; S Fuchsbichler; H Aschauer; G Kollenz; G Högenauer; F Turnowsky

Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli

J Biol Chem. 1994 Feb 25;269(8):5493-6.

Authors

H Bergler¹, P Wallner, A Ebeling, B Leitinger, S Fuchsbichler, H Aschauer, G Kollenz, G Högenauer, F Turnowsky

Affiliation

¹ Institut für Mikrobiologie, Universität Graz, Austria.

PMID: 8119879

Abstract

The EnvM protein was purified from an overproducing Escherichia coli strain. It showed NADH-dependent enoyl-acyl carrier protein (ACP) reductase activity using both crotonyl-ACP and crotonyl-CoA as substrates. The protein bound a radioactive diazaborine derivative in the presence of NAD+ and radioactive NAD+ in the presence of the drug. Based on these data, it is concluded that EnvM is the NADH-dependent enoyl-ACP reductase (EC 1.3.1.9) of E. coli and we propose to rename the corresponding gene fabI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyl Coenzyme A / metabolism
Amino Acid Sequence
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism*
Carrier Proteins / metabolism
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)
Escherichia coli / enzymology*
Escherichia coli Proteins
Fatty Acid Synthase, Type II
Kinetics
Molecular Sequence Data
NAD / metabolism
Oxidoreductases / metabolism*
Pharmaceutical Preparations / metabolism
Sequence Homology, Amino Acid

Substances

Acyl Coenzyme A
Bacterial Proteins
Carrier Proteins
Escherichia coli Proteins
Pharmaceutical Preparations
NAD
crotonyl-coenzyme A
Oxidoreductases
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)
fabI protein, E coli
Fatty Acid Synthase, Type II