Heterologous expression of endo-beta-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene

Appl Environ Microbiol. 1994 Jan;60(1):337-40. doi: 10.1128/aem.60.1.337-340.1994.

Abstract

Heterologous expression of the Clostridium cellulovorans engB gene by Clostridium acetobutylicum BKW-1 was detected as zones of hydrolysis on carboxymethyl cellulose (CMC) Trypticase glucose yeast plates stained with Congo red. The extracellular cellulase preparation from C. acetobutylicum BKW-1 has a specific activity towards CMC which is more than fourfold that present in C. acetobutylicum ATCC 824. Western blot (immunoblot) analysis using the C. cellulovorans anti-EngB primary antibody demonstrated that an additional 44-kDa protein band was present in the supernatant derived from C. acetobutylicum BKW-1 but was not present in ATCC 824 or ATCC 824(pMTL500E).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Carboxymethylcellulose Sodium / metabolism
  • Cellulase / genetics*
  • Cellulase / immunology
  • Clostridium / enzymology*
  • Clostridium / genetics*
  • Clostridium / immunology
  • Escherichia coli / genetics
  • Fermentation
  • Gene Expression
  • Genes, Bacterial*
  • Immunochemistry
  • Plasmids / genetics
  • Transformation, Genetic

Substances

  • Cellulase
  • Carboxymethylcellulose Sodium