Abstract
A plasmid vector, termed pSG5rab.gp, that expresses the rabies virus glycoprotein under the control of an SV40 early promoter, was tested in C3H/He mice for induction of rabies virus-specific immune responses. Mice immunized intramuscularly with the pSG5rab.gp vector developed rabies virus glycoprotein-specific cytolytic T cells, lymphokine-secreting T helper cells of the TH1 subset, and rabies virus-neutralizing antibodies. Mice vaccinated with the pSG5rab.gp vector were fully protected against a subsequent challenge with rabies virus.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Viral / genetics
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Antigens, Viral / immunology
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B-Lymphocytes / immunology
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Cell Line
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Cricetinae
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Female
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Genetic Vectors
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Glycoproteins / genetics*
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Glycoproteins / immunology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Plasmids / genetics*
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Plasmids / immunology
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Rabies / prevention & control*
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Rabies Vaccines / genetics*
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Rabies Vaccines / immunology
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Rabies virus / genetics*
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Rabies virus / immunology
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T-Lymphocytes / immunology
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Transfection
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / immunology
Substances
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Antigens, Viral
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Glycoproteins
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Rabies Vaccines
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Viral Envelope Proteins
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glycoprotein G, Rabies virus