Abstract
The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular / methods*
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DNA Restriction Enzymes / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Genetic Vectors*
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Molecular Sequence Data
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Plasmids
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RNA, Messenger / chemistry
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RNA, Messenger / metabolism
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Repetitive Sequences, Nucleic Acid*
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Restriction Mapping*
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Substrate Specificity
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics*
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beta-Galactosidase / metabolism
Substances
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RNA, Messenger
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DNA Restriction Enzymes
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beta-Galactosidase