Abstract
Two cDNA clones, pHSj31 and pHSj3, coding for CYP2E1 were isolated from a hamster liver cDNA library. The sequence analyses revealed that they encoded the same polypeptide of 493 amino acid residues (M(r) = 56,616) and differed from the length of their 3'-untranslated region. The deduced amino acid sequence of hamster CYP2E1 showed approx. 90% identities with those of the rats and mice, and approx. 80% identities with those of the rabbits, monkeys and humans. The NH2-terminal 35 deduced amino acid sequences of hamster CYP2E1 were completely identical with purified protein, ha P-450j. The Northern blot analysis showed that CYP2E1 was expressed in livers and to lesser extents in kidneys and lungs.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Cloning, Molecular
-
Cricetinae
-
Cytochrome P-450 CYP2E1
-
Cytochrome P-450 Enzyme System / chemistry
-
Cytochrome P-450 Enzyme System / genetics*
-
Cytochrome P-450 Enzyme System / isolation & purification
-
DNA, Complementary / chemistry
-
DNA, Complementary / isolation & purification
-
Female
-
Liver / enzymology
-
Mesocricetus
-
Molecular Sequence Data
-
Oxidoreductases, N-Demethylating / chemistry
-
Oxidoreductases, N-Demethylating / genetics*
-
Oxidoreductases, N-Demethylating / isolation & purification
Substances
-
DNA, Complementary
-
Cytochrome P-450 Enzyme System
-
Cytochrome P-450 CYP2E1
-
Oxidoreductases, N-Demethylating