Methicillin-resistant S. aureus with quinolone-resistance (QR-MRSA) isolated in Germany from three outbreaks of nosocomial infections and from sporadic nosocomial infections in five hospitals exhibited different SmaI-restriction patterns of their genomic DNA. Phage-typing and determination of plasmid profiles performed in parallel confirmed this differentiation, with one exception. These results indicate that there is obviously no overregional spread of one particular QR-MRSA clone and that quinolone resistance has developed independently in different MRSA.