Abstract
A translocation involving human chromosome 10, band q24, in a subset of T-cell acute leukemias disrupts a region surrounding the putative oncogene HOX11, which encodes a protein with a homeodomain. The HOX11 protein binds to a specific DNA sequence, it localizes to the cell nucleus, and it transactivates transcription of a reporter gene linked to a cis-regulatory element, suggesting that HOX11 functions in vivo as a positive transcription activator. PCR analysis shows that the HOX11 homeodomain is a member of a distinct class of homeodomains, representatives of which occur in murine and Drosophila genomes. These all contain a threonine residue in place of the more common isoleucine or valine in helix 3 of the homeodomain. HOX11 therefore appears to belong to a family of DNA-binding transactivators of transcription.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Cell Nucleus / metabolism
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Consensus Sequence
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DNA-Binding Proteins / genetics*
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Genes, Homeobox*
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Genes, Insect
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Homeodomain Proteins*
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Humans
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Mice
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Molecular Sequence Data
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Multigene Family
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Mutagenesis, Site-Directed
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Nuclear Proteins / genetics*
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Oligodeoxyribonucleotides / chemistry
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Oncogene Proteins / genetics*
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Proto-Oncogene Proteins
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Recombinant Proteins / metabolism
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Structure-Activity Relationship
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Transcription Factors / genetics*
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Transcriptional Activation
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Transfection
Substances
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DNA-Binding Proteins
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Homeodomain Proteins
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Oncogene Proteins
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Proto-Oncogene Proteins
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Recombinant Proteins
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Tlx1 protein, mouse
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Transcription Factors
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TLX1 protein, human
Associated data
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GENBANK/L08614
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GENBANK/L08615
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GENBANK/L08616
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GENBANK/L08617
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GENBANK/L08618
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GENBANK/L08619
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GENBANK/L08620