Cloning and restriction fragment length polymorphism analysis of a cDNA for swine PIT-1, a gene controlling growth hormone expression

Anim Genet. 1993 Feb;24(1):17-21. doi: 10.1111/j.1365-2052.1993.tb00913.x.

Abstract

A partial swine cDNA which encodes the functional domain of PIT-1 was isolated by the polymerase chain reaction (PCR). The swine PIT-1 cDNA clone is 95% identical at the protein level to the rat Pit-1 gene. Thus, Pit-1's known function in control of rat growth hormone and prolactin expression is likely to be conserved in swine. This swine cDNA clone was used to investigate genetic variability at PIT-1 in several American and Chinese breeds. Polymorphic BamHI fragments were found in pure-bred Meishan animals (n = 13), but only monomorphic fragments in five American breeds (n = 36).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Composition
  • Base Sequence
  • Blotting, Southern / veterinary
  • Cerebral Cortex / chemistry
  • Cloning, Molecular
  • DNA / isolation & purification
  • DNA-Binding Proteins / genetics*
  • Electrophoresis, Agar Gel / veterinary
  • Genes, Regulator*
  • Genetic Variation
  • Molecular Sequence Data
  • Pituitary Gland / chemistry
  • Polymerase Chain Reaction / veterinary
  • Polymorphism, Restriction Fragment Length*
  • Sequence Homology, Amino Acid
  • Swine / genetics*
  • Transcription Factor Pit-1
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Transcription Factor Pit-1
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/L03841
  • GENBANK/L06056
  • GENBANK/L20162
  • GENBANK/L20163
  • GENBANK/L20164
  • GENBANK/L20165
  • GENBANK/L20166
  • GENBANK/U10137
  • GENBANK/U10138
  • GENBANK/U10139