Abstract
A partial swine cDNA which encodes the functional domain of PIT-1 was isolated by the polymerase chain reaction (PCR). The swine PIT-1 cDNA clone is 95% identical at the protein level to the rat Pit-1 gene. Thus, Pit-1's known function in control of rat growth hormone and prolactin expression is likely to be conserved in swine. This swine cDNA clone was used to investigate genetic variability at PIT-1 in several American and Chinese breeds. Polymorphic BamHI fragments were found in pure-bred Meishan animals (n = 13), but only monomorphic fragments in five American breeds (n = 36).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Composition
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Base Sequence
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Blotting, Southern / veterinary
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Cerebral Cortex / chemistry
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Cloning, Molecular
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DNA / isolation & purification
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DNA-Binding Proteins / genetics*
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Electrophoresis, Agar Gel / veterinary
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Genes, Regulator*
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Genetic Variation
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Molecular Sequence Data
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Pituitary Gland / chemistry
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Polymerase Chain Reaction / veterinary
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Polymorphism, Restriction Fragment Length*
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Sequence Homology, Amino Acid
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Swine / genetics*
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Transcription Factor Pit-1
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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DNA-Binding Proteins
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Transcription Factor Pit-1
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Transcription Factors
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DNA
Associated data
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GENBANK/L03841
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GENBANK/L06056
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GENBANK/L20162
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GENBANK/L20163
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GENBANK/L20164
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GENBANK/L20165
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GENBANK/L20166
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GENBANK/U10137
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GENBANK/U10138
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GENBANK/U10139