Isolation of a Vibrio cholerae transposon-mutant with an altered viable but nonculturable response

J Ravel; R T Hill; R R Colwell

doi:10.1111/j.1574-6968.1994.tb07007.x

Isolation of a Vibrio cholerae transposon-mutant with an altered viable but nonculturable response

FEMS Microbiol Lett. 1994 Jul 1;120(1-2):57-61. doi: 10.1111/j.1574-6968.1994.tb07007.x.

Authors

J Ravel¹, R T Hill, R R Colwell

Affiliation

¹ Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202.

PMID: 8056295
DOI: 10.1111/j.1574-6968.1994.tb07007.x

Abstract

We have isolated more than 2500 mutants of Vibrio cholerae by using transposon mutagenesis. Mutants were screened under low nutrient conditions in artificial seawater for an altered viable but nonculturable response, compared to the wild-type. Mutant JR09H1 entered the viable but nonculturable state more rapidly than the wild-type at both 25 degrees C and 4 degrees C.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacteriological Techniques
DNA Transposable Elements / genetics*
Mutation / genetics*
Vibrio cholerae / genetics*
Vibrio cholerae / growth & development*
Vibrio cholerae / isolation & purification

Substances

DNA Transposable Elements