Enhancement of expression of human granulocyte-macrophage colony stimulating factor by argU gene product in Escherichia coli

Biochem Mol Biol Int. 1994 Mar;32(3):537-43.

Abstract

Human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned into expression vector pIN III-ompA1 and expressed in Escherichia coli JA221. When supplementation with a minor tRNA(AGA/AGG)Arg encoded by the E. coli argU gene, the expression level of hGM-CSF was raised about 3-4-fold, although there is only one rare AGG codon in hGM-CSF cDNA gene.

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Bone Marrow Cells
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Gene Expression
  • Genes, Bacterial*
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
  • Granulocyte-Macrophage Colony-Stimulating Factor / isolation & purification
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Plasmids
  • RNA, Transfer, Arg / genetics
  • RNA, Transfer, Arg / metabolism*
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology
  • Restriction Mapping

Substances

  • RNA, Transfer, Arg
  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor