Isolation of pseudorabies (Aujeszky's disease) virus from a Florida panther

C M Glass; R G McLean; J B Katz; D S Maehr; C B Cropp; L J Kirk; A J McKeirnan; J F Evermann

doi:10.7589/0090-3558-30.2.180

Isolation of pseudorabies (Aujeszky's disease) virus from a Florida panther

J Wildl Dis. 1994 Apr;30(2):180-4. doi: 10.7589/0090-3558-30.2.180.

Authors

C M Glass¹, R G McLean, J B Katz, D S Maehr, C B Cropp, L J Kirk, A J McKeirnan, J F Evermann

Affiliation

¹ Florida Game and Fresh Water Fish Commission, Wildlife Research Laboratory, Gainesville 32601.

PMID: 8028102
DOI: 10.7589/0090-3558-30.2.180

Abstract

Pseudorabies virus was isolated in cell culture from the brain tissue of a 3.5-year-old male Florida panther (Felis concolor coryi). The virus was not isolated from other tissues collected at necropsy. Based upon a nested polymerase chain reaction (PCR), the virus was determined to have the classical wild-type virulent genotype, glycoprotein I+ (gI+) and thymidine kinase+ (TK+).

Publication types

Case Reports

MeSH terms

Animals
Brain / microbiology*
Carnivora / microbiology*
DNA, Viral / analysis
Fluorescent Antibody Technique / veterinary
Genotype
Herpesvirus 1, Suid / classification
Herpesvirus 1, Suid / genetics
Herpesvirus 1, Suid / isolation & purification*
Male
Neutralization Tests / veterinary
Polymerase Chain Reaction / veterinary
Pseudorabies / microbiology*
Thymidine Kinase / genetics
Vero Cells
Viral Envelope Proteins / genetics

Substances

DNA, Viral
Viral Envelope Proteins
pseudorabies virus glycoproteins
Thymidine Kinase