Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella

Int J Biochem. 1994 Apr;26(4):575-81. doi: 10.1016/0020-711x(94)90016-7.

Abstract

1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / pharmacokinetics
  • Animals
  • Benzyl Alcohol
  • Benzyl Alcohols / pharmacology
  • Cell Membrane Permeability / drug effects
  • Cells, Cultured
  • Electron Spin Resonance Spectroscopy
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology*
  • Membrane Fluidity* / drug effects
  • Spin Labels
  • Swine

Substances

  • Albumins
  • Benzyl Alcohols
  • Spin Labels
  • Benzyl Alcohol