Abstract
The synthesis of the xylanolytic enzymes beta-xylanase and beta-xylosidase of Bacillus subtilis was studied. In contrast to many catabolic extracellular enzymes, beta-xylanase was synthesized constitutively during exponential growth and was not repressed by glucose. beta-Xylosidase synthesis was induced 100-fold by xylose and repressed 100-fold by glucose. Carbon catabolite repression was abolished in a ccpA mutant. Titration experiments using a multicopy operator sequence responsible for carbon catabolite repression indicated that the gene encoding beta-xylosidase is part of the same carbon catabolite repression regulon as the amyE and bgIS genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / drug effects
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Bacillus subtilis / genetics
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Bacillus subtilis / metabolism*
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Base Sequence
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Gene Expression Regulation, Bacterial* / drug effects
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Gene Expression Regulation, Enzymologic* / drug effects
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Glucose / pharmacology
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Glycoside Hydrolases / biosynthesis*
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Glycoside Hydrolases / genetics
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Molecular Sequence Data
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Xylan Endo-1,3-beta-Xylosidase
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Xylans / metabolism
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Xylose / metabolism
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Xylosidases / biosynthesis*
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Xylosidases / genetics
Substances
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Xylans
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Xylose
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Glycoside Hydrolases
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Xylosidases
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Xylan Endo-1,3-beta-Xylosidase
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exo-1,4-beta-D-xylosidase
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Glucose