Expression of HLA-class II genes of IDDM patients on the surface of the LTK- cells

Chin Med Sci J. 1994 Jun;9(2):108-9.

Abstract

To investigate the function of HLA-class II genes in the autoimmune response of insulin dependent diabetes mellitus (IDDM), the HLA-class II gene of IDDM patients was introduced into Ltk- cells with pSV2-neo plasmid, using the calcium phosphate precipitation technique. We obtained a stable cell line expressing the HLA-class II gene from lymphocytes of IDDM patients. Expression was identified by direct ox erythrocyte-CrCl3-HLA DR monoclonal antibody rosetting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Diabetes Mellitus, Type 1 / genetics*
  • Fibroblasts / cytology
  • Genes, MHC Class II*
  • Humans
  • Mice
  • Plasmids
  • Protein-Tyrosine Kinases / genetics
  • Transfection

Substances

  • Protein-Tyrosine Kinases