Plate fixation depends mainly on the holding power of the screws. In the present study the pattern of screw loosening was investigated. Thirty-two adult female sheep divided into four groups were used. A mid-diaphyseal transverse osteotomy was made on the right radius of each animal, and then plated on the anterior (tension) surface. Half of each group were plated using a standard narrow 7-hole AO Dynamic Compression Plate (DCP), whereas in the remaining animals a 6-hole newly designed sliding plate (SP) was applied. The required torque of tightening intraoperatively, as well as for releasing the screws after killing the animals was recorded with a tension-calibrated screwdriver. The pattern of loosening was similar for all the screws and the three phases could be recognized. An initial loosening was observed 1 month after the operation. A slow recovery of the torque was measured from the second month onwards, becoming highest by the fourth month after the operation, whereas a slow decrease in torque was observed from the fourth to the sixth month. In the SP group, the overall loosening was much lower than the DCP group (P < 0.05), whereas there was no difference in the loosening between the proximally placed screws and the distally placed ones (P > 0.05) for both plates.