Abstract
Tyrosine-hydroxylase (TH)-like immunoreactivity was investigated in normal and oestrogenized rat anterior pituitaries in the light of recent studies showing a TH enzymatic activity in the gland and the presence of TH mRNA in endocrine cells. Sparse TH positive fibres, probably dopaminergic as assessed by the absence of dopamine-beta-hydroxylase (DBH) immunoreactivity, were detected in the anterior pituitary, but the detection of TH immunoreactivity in cells was unsuccessful.
MeSH terms
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Animals
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Dopamine beta-Hydroxylase / analysis
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Estradiol / pharmacology*
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Female
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Gene Expression / drug effects
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Immunohistochemistry
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Nerve Fibers / drug effects
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Nerve Fibers / enzymology*
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Pituitary Gland, Anterior / cytology
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Pituitary Gland, Anterior / drug effects
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Pituitary Gland, Anterior / enzymology*
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Rats, Wistar
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Reference Values
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Tyrosine 3-Monooxygenase / analysis
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Tyrosine 3-Monooxygenase / biosynthesis*
Substances
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RNA, Messenger
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Estradiol
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Tyrosine 3-Monooxygenase
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Dopamine beta-Hydroxylase