Glycothiohexide alpha, a novel antibiotic produced by "Sebekia" sp., LL-14E605. II. Isolation and physical-chemical characterization

P T Northcote; D Williams; J K Manning; D B Borders; W M Maiese; M D Lee

doi:10.7164/antibiotics.47.894

Glycothiohexide alpha, a novel antibiotic produced by "Sebekia" sp., LL-14E605. II. Isolation and physical-chemical characterization

J Antibiot (Tokyo). 1994 Aug;47(8):894-900. doi: 10.7164/antibiotics.47.894.

Authors

P T Northcote¹, D Williams, J K Manning, D B Borders, W M Maiese, M D Lee

Affiliation

¹ Natural Products Research Section, American Cyanamid Company, Pearl River, New York 10965.

PMID: 7928676
DOI: 10.7164/antibiotics.47.894

Abstract

Glycothiohexide alpha was recovered from the fermentation broth of a "Sebekia" sp. by mixed solvent extraction, selective precipitation and adsorption chromatography on Diaion HP-20. The amount of glycothiohexide alpha present in the crude preparation was enriched by photolysis. Purification of glycothiohexide alpha was accomplished by repetitive countercurrent chromatography.

MeSH terms

Actinomycetales / chemistry*
Actinomycetales / metabolism
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / isolation & purification*
Chemical Phenomena
Chemistry, Physical
Circular Dichroism
Magnetic Resonance Spectroscopy
Peptides*
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet

Substances

Anti-Bacterial Agents
Peptides
glycothiohexide alpha