Abstract
The human OX-40 cell surface antigen is a CD4+ T cell activation marker that acts as a costimulatory receptor and is a member of the nerve growth factor receptor/tumor necrosis factor (TNF) receptor family. Using a soluble form of the receptor, the extracellular region fused with human immunoglobulin Fc, we expression cloned the human OX-40 ligand cDNA from a library derived from an activated B lymphoblastoid cell line MSAB. The encoded protein is identified as gp34, a type II transmembrane antigen previously known to be expressed only by human T cell lymphotropic virus 1-infected cells. We describe gp34 as a new member of the TNF family, and find that the recombinant ligand expressed in COS cells costimulates phorbol myristate acetate, phytohemagglutinin, and anti-CD3-induced CD4+ T cell proliferation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Surface / analysis*
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Antigens, Surface / chemistry
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Antigens, Surface / immunology
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Base Sequence
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CD4-Positive T-Lymphocytes / immunology*
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Cell Line, Transformed
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Cloning, Molecular
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DNA
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Humans
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Ligands
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Lymphocyte Activation
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Membrane Proteins
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Molecular Sequence Data
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Receptors, Fc / immunology
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Receptors, OX40
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Receptors, Tumor Necrosis Factor*
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Sequence Homology, Amino Acid
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Solubility
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism*
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Tumor Necrosis Factor-alpha / analysis*
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Tumor Necrosis Factor-alpha / chemistry*
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Tumor Necrosis Factor-alpha / immunology
Substances
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Antigens, Surface
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Ligands
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Membrane Proteins
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Receptors, Fc
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Receptors, OX40
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Receptors, Tumor Necrosis Factor
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TNFRSF4 protein, human
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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Tumor Necrosis Factor-alpha
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DNA