Abstract
Bone marrows from 30 newly diagnosed Ph+chronic myelocytic leukemia (CML) (21 in chronic phase, CML-CP, 9 in accelerate phase, CML-AP) and 8 followed up patients in blast crisis (CML-BC) were tested by DNA strand breaks (%D value), DNA-aneuploidy, flow cytometry-cell surface antigen expression for CD15 and HLA-DR ratio. Our results showed that all these three parameters changed as the disease escalated. A very low value of %D and DNA-aneuploidy occurrences were high risk factors. Cell surface antigen expression CD15 and HLA-DR ratio measurement was simple and reliable. The ratio < 1.0 appeared earlier than morphology clarifying CML-AP and should be followed up regularly.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aneuploidy
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Antigens, CD / metabolism
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Antigens, Differentiation, Myelomonocytic / metabolism
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Biomarkers, Tumor / metabolism
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Blast Crisis / genetics
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Blast Crisis / immunology
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Blast Crisis / pathology
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DNA Damage
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism*
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Flow Cytometry
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HLA-DR Antigens / metabolism
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / immunology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology*
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Lewis X Antigen
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Prognosis
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Risk Factors
Substances
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Antigens, CD
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Antigens, Differentiation, Myelomonocytic
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Biomarkers, Tumor
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DNA, Neoplasm
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HLA-DR Antigens
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Lewis X Antigen