The distribution of the alpha-N-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the Pacific Ocean and 423 Baikalian microorganisms from fresh water and sponges have been studied. About 24% as marine as Baikalian strains produced this enzyme. The most number of alpha-N-Ac-galactosidase producers were found for marine bacteria genera Aeromonas, Alteromonas and Vibrio (71% and by 60%, respectively) and Baikalian microorganisms genera Aeromonas and Bacillus (40% and 31%, respectively). alpha-N-Acetyl-galactosidase was not found for marine arthrobacters, Flexibacter-Cytophaga and baikalian Micrococcus and Rhodococcus. The promising sources of the alpha-N-Ac-galactosidases were found among Alteromonas and Bacillus.