Ebulitins: a new family of type 1 ribosome-inactivating proteins (rRNA N-glycosidases) from leaves of Sambucus ebulus L. that coexist with the type 2 ribosome-inactivating protein ebulin 1

F M de Benito; L Citores; R Iglesias; J M Ferreras; F Soriano; J Arias; E Méndez; T Girbés

doi:10.1016/0014-5793(95)00130-2

Ebulitins: a new family of type 1 ribosome-inactivating proteins (rRNA N-glycosidases) from leaves of Sambucus ebulus L. that coexist with the type 2 ribosome-inactivating protein ebulin 1

FEBS Lett. 1995 Mar 6;360(3):299-302. doi: 10.1016/0014-5793(95)00130-2.

Authors

F M de Benito¹, L Citores, R Iglesias, J M Ferreras, F Soriano, J Arias, E Méndez, T Girbés

Affiliation

¹ Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, Spain.

PMID: 7883051
DOI: 10.1016/0014-5793(95)00130-2

Abstract

A new family of single chain (type 1) ribosome-inactivating proteins (RIPs), that we have named ebulitins, have been found in mature leaves of Sambucus ebulus L., a caprifoliaceae plant also known to contain a non-toxic two chain (type 2) RIP named ebulin I in its leaves. Ebulitins are basic proteins of M(r) 32,000, 29,000 and 29,000 for ebulitins alpha, beta and gamma, respectively. The simultaneous presence of different basic type 1 and acidic type 2 RIPs in the same plant and in the same tissue is described here for the first time and opens a new door in research into RIPs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / analysis
Glycoside Hydrolases / isolation & purification*
Molecular Weight
N-Glycosyl Hydrolases*
Plant Proteins / chemistry
Plant Proteins / isolation & purification*
Plant Proteins / pharmacology
Protein Synthesis Inhibitors / isolation & purification*
RNA, Ribosomal / metabolism
Ribosome Inactivating Proteins, Type 2
Ribosomes / drug effects

Substances

Amino Acids
Plant Proteins
Protein Synthesis Inhibitors
RNA, Ribosomal
Ribosome Inactivating Proteins, Type 2
Glycoside Hydrolases
N-Glycosyl Hydrolases