High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides

H Karlsson; N Karlsson; G C Hansson

doi:10.1007/BF02921557

High-temperature gas chromatography and gas chromatography-mass spectrometry of glycoprotein and glycosphingolipid oligosaccharides

Mol Biotechnol. 1994 Apr;1(2):165-80. doi: 10.1007/BF02921557.

Authors

H Karlsson¹, N Karlsson, G C Hansson

Affiliation

¹ University of Göteborg, Department of Medical Biochemistry, Sweden.

PMID: 7859159
DOI: 10.1007/BF02921557

Abstract

High-temperature gas chromatography and gas chromatography-mass spectrometry for the analyses of oligosaccharides derived from glycoproteins or glycosphingolipids has been developed. Permethylated oligosaccharides with up to about 12 sugar residues and masses up to 2500 Daltons can be analyzed. This approach is discussed and exemplified.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biotechnology
Carbohydrate Sequence
Chromatography, Gas / methods*
Gas Chromatography-Mass Spectrometry / methods*
Glycoconjugates / chemistry*
Glycopeptides / chemistry
Glycoproteins / chemistry
Glycosphingolipids / chemistry
Hot Temperature
Molecular Sequence Data
Oligosaccharides / analysis*
Oligosaccharides / chemistry
Polysaccharides / chemistry
Sialic Acids / chemistry

Substances

Glycoconjugates
Glycopeptides
Glycoproteins
Glycosphingolipids
Oligosaccharides
Polysaccharides
Sialic Acids