A small, high-copy-number vector suitable for both in vitro and in vivo gene expression

Gene. 1994 Dec 30;151(1-2):143-5. doi: 10.1016/0378-1119(94)90645-9.

Abstract

A 2433-bp, high-copy-number expression vector, pUK, was constructed from pUC19 and pKK223-3. The genes encoding chicken adenylate kinase (AK) and phospholipase A2, cloned into pUK, were highly expressed in vitro using the coupled transcription/translation system of Zubay. High-level in vivo expression in Escherichia coli was also demonstrated for the AK gene cloned in this vector. The multiple cloning site (MCS) of pKK223-3 was preserved in pUK. In addition, three more sites in the MCS sequence, AccI, BamHI and SalI, were made unique for the convenience of cloning.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylate Kinase / biosynthesis
  • Adenylate Kinase / genetics*
  • Adenylate Kinase / isolation & purification
  • Animals
  • Base Sequence
  • Chickens
  • Cloning, Molecular / methods*
  • Escherichia coli
  • Gene Expression*
  • Genetic Vectors*
  • Molecular Sequence Data
  • Phospholipases A / biosynthesis
  • Phospholipases A / genetics*
  • Phospholipases A / isolation & purification
  • Phospholipases A2
  • Plasmids
  • Protein Biosynthesis
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Restriction Mapping
  • Transcription, Genetic

Substances

  • Recombinant Proteins
  • Adenylate Kinase
  • Phospholipases A
  • Phospholipases A2