Abstract
A 2433-bp, high-copy-number expression vector, pUK, was constructed from pUC19 and pKK223-3. The genes encoding chicken adenylate kinase (AK) and phospholipase A2, cloned into pUK, were highly expressed in vitro using the coupled transcription/translation system of Zubay. High-level in vivo expression in Escherichia coli was also demonstrated for the AK gene cloned in this vector. The multiple cloning site (MCS) of pKK223-3 was preserved in pUK. In addition, three more sites in the MCS sequence, AccI, BamHI and SalI, were made unique for the convenience of cloning.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenylate Kinase / biosynthesis
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Adenylate Kinase / genetics*
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Adenylate Kinase / isolation & purification
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Animals
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Base Sequence
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Chickens
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Cloning, Molecular / methods*
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Escherichia coli
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Gene Expression*
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Genetic Vectors*
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Molecular Sequence Data
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Phospholipases A / biosynthesis
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Phospholipases A / genetics*
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Phospholipases A / isolation & purification
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Phospholipases A2
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Plasmids
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Protein Biosynthesis
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Restriction Mapping
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Transcription, Genetic
Substances
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Recombinant Proteins
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Adenylate Kinase
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Phospholipases A
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Phospholipases A2