DBA/2Ibg mice were treated with cholesterol pellets for 11 days. On the seventh day after treatment, animals began 5 consecutive days of training on the spatial form of Morris water task, followed on the third and fourth days by a probe trial, and random platform training on the fifth day. DBA mice with cholesterol pellets exhibited enhanced performance compared to DBA mice that underwent a sham surgery. Our results suggest that subchronic treatment with the steroid hormone precursor, cholesterol, enhances spatial learning performance in DBA mice.