Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR

J Clin Microbiol. 1994 Oct;32(10):2581-3. doi: 10.1128/jcm.32.10.2581-2583.1994.

Abstract

The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / blood*
  • Blood Specimen Collection
  • Cytomegalovirus / drug effects
  • Cytomegalovirus / isolation & purification*
  • Edetic Acid / pharmacology*
  • Heparin / pharmacology*
  • Humans
  • Leukocytes / virology*
  • Phosphoproteins / blood*
  • Polymerase Chain Reaction*
  • Viral Matrix Proteins / blood*

Substances

  • Antigens, Viral
  • Phosphoproteins
  • Viral Matrix Proteins
  • cytomegalovirus matrix protein 65kDa
  • Heparin
  • Edetic Acid