Abstract
The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Viral / blood*
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Blood Specimen Collection
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Cytomegalovirus / drug effects
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Cytomegalovirus / isolation & purification*
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Edetic Acid / pharmacology*
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Heparin / pharmacology*
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Humans
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Leukocytes / virology*
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Phosphoproteins / blood*
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Polymerase Chain Reaction*
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Viral Matrix Proteins / blood*
Substances
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Antigens, Viral
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Phosphoproteins
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Viral Matrix Proteins
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cytomegalovirus matrix protein 65kDa
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Heparin
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Edetic Acid