Characterization and protective effect of a 29 kDa protein isolated from Coxiella burnetii by detergent Empigen BB

M Lukácová; E Gajdosová; L Skultéty; E Kovácová; J Kazár

doi:10.1007/BF01730376

Characterization and protective effect of a 29 kDa protein isolated from Coxiella burnetii by detergent Empigen BB

Eur J Epidemiol. 1994 Apr;10(2):227-30. doi: 10.1007/BF01730376.

Authors

M Lukácová¹, E Gajdosová, L Skultéty, E Kovácová, J Kazár

Affiliation

¹ Institute of Virology, Slovak Academy of Sciences, Bratislava.

PMID: 7813704
DOI: 10.1007/BF01730376

Abstract

A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates a strong binding between proteins and LPS in the outer membrane of C. burnetii. The protein was immunogenic in mice and protected them against virulent C. burnetii challenge.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibody Formation
Bacterial Outer Membrane Proteins / analysis*
Bacterial Outer Membrane Proteins / physiology
Blotting, Western
Coxiella burnetii / chemistry*
Coxiella burnetii / classification
Coxiella burnetii / immunology
Coxiella burnetii / pathogenicity
Detergents*
Disease Models, Animal
Electrophoresis, Gel, Two-Dimensional
Lipopolysaccharides / metabolism
Mice
Mice, Inbred BALB C
Organic Chemicals
Protein Binding

Substances

Bacterial Outer Membrane Proteins
Detergents
Lipopolysaccharides
Organic Chemicals
empigen BB