Molecular analysis of two Brassica napus genes expressed in the stigma

Plant Mol Biol. 1994 Nov;26(4):1217-22. doi: 10.1007/BF00040703.

Abstract

A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Brassica / anatomy & histology
  • Brassica / genetics*
  • Brassica / growth & development
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Genes, Plant / genetics*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Plant Shoots / anatomy & histology
  • Plant Shoots / genetics*
  • Plant Shoots / growth & development
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution

Substances

  • DNA, Complementary

Associated data

  • GENBANK/L34287
  • GENBANK/L34288