Abstract
A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Brassica / anatomy & histology
-
Brassica / genetics*
-
Brassica / growth & development
-
Cloning, Molecular
-
DNA, Complementary / genetics
-
Gene Expression
-
Genes, Plant / genetics*
-
In Situ Hybridization
-
Molecular Sequence Data
-
Plant Shoots / anatomy & histology
-
Plant Shoots / genetics*
-
Plant Shoots / growth & development
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
-
Tissue Distribution
Associated data
-
GENBANK/L34287
-
GENBANK/L34288