The aim of this study was to investigate heart rate threshold (HRT) related exercise intensities by means of two endurance cycle ergometer tests using blood lactate concentration [La], pulmonary ventilation (VE), oxygen uptake (VO2), heart rate (HR) and electromyogram (EMG) activity of working muscle. Firstly, 16 healthy female students [age, 21.4 (SD 2.8) years; height, 167.1 (SD 5.1) cm; body mass 62.7 (SD 7.1) kg] performed an incremental exercise test (10 W each minute) on an electrically braked cycle ergometer until they felt exhausted. The HRT and lactate turn point (LTP) were assessed by means of computer-aided linear regression break point analysis from the relationship of HR or [La] to power output. No significant difference was found between HRT and LTP for all the variables measured. Secondly, two endurance tests (ET) of 20 min duration were performed by 7 subjects. The first (ET I) was performed at an exercise intensity which was about 10% lower than the power output at HRT [61.2 (SD 3.1)% maximal oxygen uptake (VO2max)], the second (ET II) at an exercise intensity about 10% higher than the power output at HRT [79.2 (SD 3.4) % VO2max]. The parameters measured showed a clear steady state in ET I. All mean values were lower than values at HRT [power, 138.7 (SD 18.9) W; HR, 172.1 (SD 4.7) beats.min-1; VO2, 2.2 (SD 0.3) l.min-1; VE, 54.0 (SD 9.1) l.min-1; [La], 3.7 (SD 1.1) mmol.l-1; EMG, 81.1 (SD 24.0) microV] except HR which was the same.(ABSTRACT TRUNCATED AT 250 WORDS)