Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes teichomyceticus leuC gene

Gene. 1995 May 26;158(1):97-100. doi: 10.1016/0378-1119(95)00142-s.

Abstract

A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase (IPMI), and the 5' end of a second ORF corresponding to leuD, which encodes the smaller subunit of IPMI. Further subcloning established that Sl strain PC196 is defective in the large subunit of IPMI.

MeSH terms

  • Actinomycetaceae / genetics*
  • Bacterial Proteins / genetics*
  • Cloning, Molecular
  • Genes, Bacterial*
  • Genetic Complementation Test*
  • Hydro-Lyases*
  • Isomerases / genetics*
  • Molecular Sequence Data
  • Mutation*
  • Streptomyces / genetics*

Substances

  • Bacterial Proteins
  • Hydro-Lyases
  • LeuC protein, bacteria
  • isopropylmalate isomerase
  • Isomerases

Associated data

  • GENBANK/X84647