Abstract
1. The effects of restraint stress on c-fos mRNA expression in the dentate gyrus were investigated by in situ hybridization. 2. Confirming previous findings, c-fos mRNA expression increased after 30 min of forced restraint. 3. This effect was attenuated by a previous i.c.v. injection of the anxiolytic benzodiazepine midazolam (20 nmol/2 microliters) or the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid (AP-7; 5 nmol/2 microliters). 4. These results suggest that the dentate gyrus is activated during restraint stress and that this activation may be modulated by benzodiazepine gamma-aminobutyric acidA (GABAA) or NMDA receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Amino-5-phosphonovalerate* / analogs & derivatives*
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Amino Acids / pharmacology*
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Animals
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Gene Expression Regulation / drug effects*
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Genes, fos*
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Hippocampus / drug effects*
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Hippocampus / metabolism
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Hippocampus / physiopathology
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Immobilization
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In Situ Hybridization
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Male
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Midazolam / pharmacology*
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RNA, Messenger / biosynthesis*
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RNA, Messenger / genetics
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Rats
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Rats, Wistar
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Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors*
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Receptors, N-Methyl-D-Aspartate / physiology
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Stress, Physiological / genetics*
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Stress, Physiological / metabolism
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Stress, Physiological / physiopathology
Substances
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Amino Acids
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RNA, Messenger
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Receptors, N-Methyl-D-Aspartate
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2-Amino-5-phosphonovalerate
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2-amino-7-phosphonoheptanoic acid
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Midazolam