Midazolam and the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid (AP-7) attenuate stress-induced expression of c-fos mRNA in the dentate gyrus

Cell Mol Neurobiol. 1994 Aug;14(4):373-80. doi: 10.1007/BF02088717.

Abstract

1. The effects of restraint stress on c-fos mRNA expression in the dentate gyrus were investigated by in situ hybridization. 2. Confirming previous findings, c-fos mRNA expression increased after 30 min of forced restraint. 3. This effect was attenuated by a previous i.c.v. injection of the anxiolytic benzodiazepine midazolam (20 nmol/2 microliters) or the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid (AP-7; 5 nmol/2 microliters). 4. These results suggest that the dentate gyrus is activated during restraint stress and that this activation may be modulated by benzodiazepine gamma-aminobutyric acidA (GABAA) or NMDA receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Amino-5-phosphonovalerate* / analogs & derivatives*
  • Amino Acids / pharmacology*
  • Animals
  • Gene Expression Regulation / drug effects*
  • Genes, fos*
  • Hippocampus / drug effects*
  • Hippocampus / metabolism
  • Hippocampus / physiopathology
  • Immobilization
  • In Situ Hybridization
  • Male
  • Midazolam / pharmacology*
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors*
  • Receptors, N-Methyl-D-Aspartate / physiology
  • Stress, Physiological / genetics*
  • Stress, Physiological / metabolism
  • Stress, Physiological / physiopathology

Substances

  • Amino Acids
  • RNA, Messenger
  • Receptors, N-Methyl-D-Aspartate
  • 2-Amino-5-phosphonovalerate
  • 2-amino-7-phosphonoheptanoic acid
  • Midazolam