Abstract
cDNA clones of a tomato protein, called Lycopersicum esculentum putative Mg(2+)-dependent ATPase (LeMA-1), were isolated from a cDNA library. Sequence comparison of the tomato protein with other genes in the database revealed that the protein is highly homologous to a human protein called TBP-1 and a yeast Tat-binding-analogue protein YTA1A. All three proteins belong to the recently discovered protein family of putative Mg(2+)-dependent ATPases and form within this family a subgroup of proteins involved in controlled protein degradation and possibly also in transcriptional regulation. Expression of the mRNA of LeMA-1 could be monitored in several plant tissues. LeMA-1 is the first member of this subgroup of proteins isolated from plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATPases Associated with Diverse Cellular Activities
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Adenosine Triphosphatases / genetics*
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Amino Acid Sequence
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Ca(2+) Mg(2+)-ATPase / chemistry
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Ca(2+) Mg(2+)-ATPase / genetics*
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Carrier Proteins / genetics
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Cell Cycle Proteins / genetics
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Fungal Proteins / genetics
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Humans
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Iron-Binding Proteins
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Membrane Proteins / genetics
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Molecular Sequence Data
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Plant Leaves / metabolism
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Solanum lycopersicum / genetics*
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Transferrin-Binding Proteins
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Valosin Containing Protein
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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Cell Cycle Proteins
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Fungal Proteins
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Iron-Binding Proteins
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Membrane Proteins
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Plant Proteins
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Saccharomyces cerevisiae Proteins
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Transferrin-Binding Proteins
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Vesicular Transport Proteins
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Adenosine Triphosphatases
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Ca(2+) Mg(2+)-ATPase
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LeMA-1 protein, Lycopersicum esculentum
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SEC18 protein, S cerevisiae
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ATPases Associated with Diverse Cellular Activities
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CDC48 protein, S cerevisiae
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PEX1 protein, S cerevisiae
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Valosin Containing Protein