Abstract
The effect of insulin on polypeptide chain elongation was examined in soleus muscles isolated from 18 hour-fasted mice. Treatment with insulin for 1 hour increased the elongation rate, which was estimated by the half-transit time. This suggests that insulin stimulated protein synthesis by modifying the elongation rate in addition to the initiation rate.
MeSH terms
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Animals
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Diabetes Mellitus, Experimental / metabolism
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Electrophoresis, Polyacrylamide Gel
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Insulin / administration & dosage
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Insulin / pharmacology*
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Male
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Mice
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Muscle Proteins / biosynthesis*
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Muscle, Skeletal / drug effects*
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Muscle, Skeletal / metabolism
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Nerve Tissue Proteins / metabolism
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Peptide Biosynthesis
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Peptide Chain Elongation, Translational / drug effects*
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Peptide Elongation Factor 1
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Peptide Elongation Factor 2
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Peptide Elongation Factors / metabolism
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Phosphorylation
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Ribonucleoproteins / metabolism
Substances
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Insulin
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Muscle Proteins
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Nerve Tissue Proteins
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Peptide Elongation Factor 1
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Peptide Elongation Factor 2
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Peptide Elongation Factors
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Ribonucleoproteins