Abstract
An M-like protein from Streptococcus pyogenes type M15 strain EF1949 (EMML15) was cloned in Escherichia coli and sequenced. Recombinant EMML15 protein revealed a unique binding pattern for human IgG subclasses not described previously. Comparative analysis of the EMML15 amino acid sequence with those of other M-like proteins of opacity factor positive (OF+) serotypes and protein H, and IgG receptor from OF- serotype M1, showed that IgG-binding proteins with common binding of IgG3 were closely related and distinct from streptococcal IgG receptors not binding IgG3. Thus, the Ig-binding proteins from S. pyogenes were subdivided into two main categories according to binding pattern, protein structure, and gene location.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Bacterial*
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Bacterial Outer Membrane Proteins*
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Bacterial Proteins / classification
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Carrier Proteins*
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Cloning, Molecular
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Consensus Sequence
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DNA, Bacterial / analysis
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Genes, Bacterial / genetics*
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Immunoglobulin G / metabolism*
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Molecular Sequence Data
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Protein Binding
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Receptors, IgG / genetics
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Receptors, IgG / metabolism
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Recombinant Proteins / genetics
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Sequence Analysis, DNA
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Streptococcus pyogenes / genetics*
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Carrier Proteins
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DNA, Bacterial
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Immunoglobulin G
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Receptors, IgG
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Recombinant Proteins
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streptococcal M protein