Isozyme specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin peroxidase genes of Phanerochaete chrysosporium

P Brooks; P Sims; P Broda

doi:10.1038/nbt0793-830

Isozyme specific polymerase chain reaction analysis of differential gene expression: a general method applied to lignin peroxidase genes of Phanerochaete chrysosporium

Biotechnology (N Y). 1993 Jul;11(7):830-4. doi: 10.1038/nbt0793-830.

Authors

P Brooks¹, P Sims, P Broda

Affiliation

¹ Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.

PMID: 7763862
DOI: 10.1038/nbt0793-830

Abstract

Analysis of differential expression of closely related genes is a general problem. The lignin peroxidase genes of Phanerochaete chrysosporium represent a typical case. They are differentially expressed according to the conditions encountered during growth. We show that the expression of two such genes, LIG1 and LIG5, can be differentiated at the mRNA level using a highly specific form of the polymerase chain reaction. This generally applicable method allows rapid and accurate analysis of complex and closely related mRNA populations.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Culture Media
DNA, Fungal / genetics*
DNA, Single-Stranded
Gene Expression*
Genes, Fungal*
Introns
Isoenzymes / genetics*
Molecular Sequence Data
Peroxidases / genetics*
Polymerase Chain Reaction
RNA, Fungal / genetics*
RNA, Messenger / genetics
Templates, Genetic

Substances

Culture Media
DNA, Fungal
DNA, Single-Stranded
Isoenzymes
RNA, Fungal
RNA, Messenger
Peroxidases
lignin peroxidase